Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: PPY-Induced iCAFs Cultivate an Immunosuppressive Microenvironment in Pancreatic Cancer.

doi: 10.1002/advs.202413432

Figure Lengend Snippet: Figure 1. Screening and initial validation of cancer cell-secreted proteins capable of significantly inducing iCAF phenotype. A) The t-distributed stochastic neighbor embedding (t-SNE) plot of the 92,222 cells in the single-cell sequencing profile revealed distinct cell types observed in PDAC. B) The t-SNE plot exhibited diverse subtypes of fibroblasts observed in PDAC. C) The top 10 up-regulated and down-regulated expressed marker genes of each CAF subgroup. D) The expression levels of myCAF markers (ACTA2, COL1A1, COL11A1, MMP11), iCAF markers (CXCL12, IL-6, CCL2, CXCL2), and apCAF markers (HLA-DRA, HLA-DRB1) in different fibroblast subsets. E) Pathway activities scored by GSVA between different fibroblast subsets. F) The volcano

Article Snippet: ELISA assays used were Mouse CXCL12 ELISA kit (KE10049, Proteintech), Mouse IL-6 ELISA kit (EK206HS, MULTI SCIENCES), Mouse CCL2 ELISA kit (EK287, MULTI SCIENCES), Human CXCL12 ELISA kit (EK1119, MULTI SCIENCES), Human CCL2 ELISA kit (EK187, MULTI SCIENCES), and Human IL-6 ELISA kit (EK106, MULTI SCIENCES).

Techniques: Biomarker Discovery, Sequencing, Marker, Expressing

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: PPY-Induced iCAFs Cultivate an Immunosuppressive Microenvironment in Pancreatic Cancer.

doi: 10.1002/advs.202413432

Figure Lengend Snippet: Figure 2. PPY significantly induces the iCAF phenotype in PDAC CAFs both in vitro and in vivo. A–C) After treating CAFs derived from human PDAC tissues for 12 h, qRT-PCR analysis was performed to assess their alterations in the expression of iCAF markers (CXCL12 (A), IL-6 (B), CXCL12 (C)). D–F) qRT-PCR analysis of iCAF markers (CXCL12 (D), IL-6 (E), CXCL12 (F)) after treating the human CAFs for 24 h. G–I) qRT-PCR analysis of iCAF markers (CXCL12 (G), IL-6 (H), CXCL12 (I)) after treating the human CAFs for 36 h. J) qRT-PCR analysis of the expression levels of myCAF markers (ACTA2 and CTGF) after treating the human CAFs with PPY proteins (40ng/ml) for 24 h. K) Flow cytometry analysis was performed to evaluate the

Article Snippet: ELISA assays used were Mouse CXCL12 ELISA kit (KE10049, Proteintech), Mouse IL-6 ELISA kit (EK206HS, MULTI SCIENCES), Mouse CCL2 ELISA kit (EK287, MULTI SCIENCES), Human CXCL12 ELISA kit (EK1119, MULTI SCIENCES), Human CCL2 ELISA kit (EK187, MULTI SCIENCES), and Human IL-6 ELISA kit (EK106, MULTI SCIENCES).

Techniques: In Vitro, In Vivo, Derivative Assay, Quantitative RT-PCR, Expressing, Flow Cytometry

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: PPY-Induced iCAFs Cultivate an Immunosuppressive Microenvironment in Pancreatic Cancer.

doi: 10.1002/advs.202413432

Figure Lengend Snippet: Figure 7. The inhibition of EGFR expression in CAFs impeded the induction of iCAFs by PPY. A) qRT-PCR (A) and B) ELISA analyses of the expression levels of IL-6, CCL2, and CXCL12 in human EGFR-knockdown CAFs treated with PPY proteins. C) The efficiency of EGFR knockdown in KPC CAFs was examined by qRT-PCR. D,E) qRT-PCR (D) and ELISA (E) analyses of the expression levels of IL-6, CCL2, and CXCL12 in murine EGFR knockdown CAFs treated with PPY proteins. F) Flow cytometry analysis was performed to evaluate the populations of iCAFs, myCAFs, and apCAFs in murine EGFR- knockdown CAFs treated with PPY proteins. G,H) The IVIS image (G) and gross image (H) of tumors in model mice (n = 7), that was constructed

Article Snippet: ELISA assays used were Mouse CXCL12 ELISA kit (KE10049, Proteintech), Mouse IL-6 ELISA kit (EK206HS, MULTI SCIENCES), Mouse CCL2 ELISA kit (EK287, MULTI SCIENCES), Human CXCL12 ELISA kit (EK1119, MULTI SCIENCES), Human CCL2 ELISA kit (EK187, MULTI SCIENCES), and Human IL-6 ELISA kit (EK106, MULTI SCIENCES).

Techniques: Inhibition, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Knockdown, Flow Cytometry, Construct

Journal: Stem Cell Research & Therapy

Article Title: Establishment of human hematopoietic organoids for evaluation of hematopoietic injury and regeneration effect

doi: 10.1186/s13287-024-03743-y

Figure Lengend Snippet: Construction of the in vitro radiation injury model based on the hematopoietic organoids. a Schematic diagram demonstrates the organoids from -Niche cell and + Niche cell groups received radiation at 0 or 4 Gy. b Comparative analysis of CXCL12 concentration in the two groups under different conditions one day after radiation exposure. Results are presented as the mean ± SD from three independent experiments. One-way ANOVA, ** p < 0.01; *** p < 0.001;### p < 0.001. c Flow cytometry analysis of the percentage of caspase-3-positive cells in CD45 + cell population from the two groups one day after radiation exposure. Results are presented as the mean ± SD from three independent experiments. One-way ANOVA, *** p < 0.001. d Flow cytometry analysis of the percentage of caspase-3-positive cells in CD34 + CD45 + cell population from the two groups one day after radiation exposure. Results are presented as the mean ± SD from three independent experiments. One-way ANOVA, *** p < 0.001. e Percentage of CD34 + CD45 + cells on the first and fourth day after 4 Gy irradiation. Results are presented as the mean ± SD from three independent experiments. One-way ANOVA, *** p < 0.001. f Schematic diagram of the construction of the radiation injury model. g ROS levels in CD45 + and CD34 + CD45 + cells under different radiation doses. Results are presented as the mean ± SD from three independent experiments. One-way ANOVA, * p < 0.05; ** p < 0.01; *** p < 0.001. h γ-H2AX expression levels in CD45 + cells under different radiation doses. Results are presented as the mean ± SD from three independent experiments. One-way ANOVA, * p < 0.05. I. γ-H2AX expression levels in CD34 + CD45 + cells under different radiation doses. Results are presented as the mean ± SD from three independent experiments. One-way ANOVA, * p < 0.05

Article Snippet: The levels of CXCL12 in the cells were detected by using Human CXCL12 ELISA Kit (BOSTER, Wuhan, China) according to the manufacturer’s instructions.

Techniques: In Vitro, Concentration Assay, Flow Cytometry, Irradiation, Expressing